Publications


Maximova SN, Florez S, Xiangling Shen X, Niemenak N, Zhang Y, Curtis WR, Guiltinan MJ (2014)  Genome-wide analysis reveals divergent patterns of geneexpression during zygotic and somatic embryo maturation of Theobroma cacao L., thechocolate treeBMC Plant Biology 2014, 14:185  doi:10.1186/1471-2229-14-185.


Zuroff TR, Weimin Gu W, Fore RL, Leschine SB, Curtis WR (2014) Insights into Clostridium phytofermentans biofilm formation: aggregation, micro-colony development and the role of extracellular DNA, Mircrobiology, 160(6): 1134-1143. 

Curtis WR, Curtis MS (2014) Biomass-2-Energy, Chapter 3, In: Systems Engineering for Clean and Renewable Energy Manufacturing in Europe and Asia, NSF sponsored REport, WTEC.  (Full Report on Web; http://wtec.org/SEEM/) 

Erbakan M, Shen Y-x, Butler PJ, Grelakowski M, Kumar M, Curtis WR (2014) Molecular cloning, overexpression and characterization of a novel water channel protein from Rhodobacter sphaeroides. PLoS1, DOI: 10.1371/journal.pone.0086830


Khatri W, Hendrix R, Niehaus T, Chappell J, Curtis WR (2013) Hydrocarbon Production in High Density Botryococcus braunii Race B Continuous Culture, Biotechnology & Bioengineering, 111 (3), 493-503doi:10.1002/bit.25126
Zuroff TR, Salvador Barri Xiques SB, Curtis WR (2013) Consortia-mediated bioprocessing of cellulose to ethanol with a symbiotic Clostridium phytofermentans/yeast co-culture. Biotechnology for Biofuels, 6:59  doi:10.1186/1754-6834-6-59

Myers JA, Curtis BS, Curtis WR (2013) Improving accuracy of cell and chromophore concentration measurements using optical density. BMC Biophysics 2013, 6:4, 1-16.    

Zuroff TR, Xiques SB, Curtis WR (2013Consortia-mediated bioprocessing of cellulose to ethanol with a symbiotic Clostridium phytofermentans/yeast co-culture, Biotechnology for Biofuels, 6:59  doi:10.1186/1754-6834-6-59


Larsen, J.S.; Curtis W.R. (2012) RNA viral vectors for improved Agrobacterium-mediated transient expression of heterologous proteins in Nicotiana benthamiana cell suspensions and hairy roots. BMC Biotechnology 2012, 12:21.

Zuroff, Trevor, Curtis, WR. (2012)  Developing symbiotic consortia for lignocellulosic biofuel production.  Applied Microbiology and Biotechnology. 2012 Feb ;93(4):1423-35. Epub 2012 Jan 26  Doi: 10.1007/s00253-011-3762-9

Woolston BM, Schlagnhaufer C, Wilkinson J, Larsen J, Shi Z, Mayer K, Waters D, Curtis W, Romaine C (2011) Long-Distance Translocation of Protein during Morphogenesis of the Fruiting Body in the Filamentous Fungus, Agaricus bisporus. PLoS ONE 6(12): e28412,  doi:10.1371/journal.pone.0028412 


Aziz A, Werner BC, Epting KL, Agosti CD, Curtis WR (2007)  The cumulative and sublethal effects of turbulence on erythrocytes in a stirred-tank model.  Annals of Biomedical Engineering 35(12):2108-2120. 

Collens JI, Mason HS, Curtis, WR (2007) Agrobacterium-mediated viral vector-amplified transient gene expression in Nicotiana glutinosa plant tissue culture. Biotechnol Prog. May-Jun;23(3):570-576.

Curtis, W.R., Tuerk, Amalie L. 2006. Oxygen Transport in Plant Tissue Culture Systems. In: S.Dutta Gupta and Y.Ibaraki (Eds) Plant Tissue Culture Engineering, Focus on Biotechnology Vol.6., Springer, Dordrecht; pp:173-186.

Andrews, Lauren B., Curtis, W.R.  Comparison of transient protein expression in tobacco leaves and plant suspension culture. Biotechnol. Prog. 21(3):946-952, 2005.

Curtis WR (2005)  Protein Production in Transgenic Plants. In: Encyclopedia of Chemical Processing, vol. 4 (Lee S, ed.), Taylor & Francis, NY, pp. 2489-2500.  (Feel free to email the author to request a desk copy).

Nitin Kumar, Wayne Curtis, and Jong-in Hahm, Laterally aligned, multiwalled carbon nanotube growth using Magnetospirillium magnetotacticum  Appl. Phys. Lett. 86, 173101-173103,  2005.  

Curtis, W.R.  Application of bioreactor design principles to plant micropropagation. Plant Cell, Tissue and Organ Culture, 81(3):255-264, 2005. 

Curtis, W.R.  Application of bioreactor design principles to plant micropropagation.  Chapter 2: Liquid Systems for in Vitro Plant Propagation (Hvoslef-Eide, K., Preil, W. eds), Springer, pp. 21-40, 2005.

Divakar Ramakrishnan, Curtis, W.R.  Trickle-bed root culture bioreactor design and scale-up: Growth, fluid-dynamics, and oxygen mass transfer.  Biotechnology & Bioegineering, 88(2):248-260, 2004.  

Collens, J.I., Lee, D.R., Seeman, A.M. Seeman, Curtis, W.R. The Development of Auxotrophic Agrobacterium tumefaciens for Gene Transfer in Plant Tissue Culture. Biotechnol. Prog. 20(3); 890-896, 2004. DOI=10.1021/bp034306w

Curtis, W.R., Quantitative transient protein expression in plant tissue culture, U.S. Patent # 6,740,526, May 25, 2004.

Curtis, W.R., Growing cells in a reservoir formed of a flexible sterile plastic liner, U.S. Patent # 6,709,862, March 23, 2004.

Cuello JL, P. N. Walker, Curtis WR, Design of ebb-and-flow bioreactor (EFBR) for immobilized "hairy root" cultures:  Part II. Growth studies and model verification.  Transactions of the ASAE, 46(5): 1469–1476, 2003.  

Curtis, W.R.,  Method and apparatus for aseptic growth or processing of biomass, U.S. Patent # 6,245,555, June 12, 2001.

Curtis, W.R.; Carvalho, E.B., Effect of elicitation on growth, respiration and nutrient uptake of root and cell suspension cultures of Hyoscyamus muticus". Biotechnology Progress, 18:282-289, 2002. DOI=10.1021/bp0202970

Asplund, T.A.; Curtis, W.R., Intrinsic oxygen use kinetics of transformed root culture, Biotechnology Progress, 17:481-489, 2001.

Mukundan, Usha; Bhagwat, Veena; Singh, Gurmeet; Curis, W.R. Integrated recovery of pigments released from red beet hairy roots exposed to acidic medium. J. Plant Biochem. Biotechnol. 10:67-69, 2001.

Bordonaro, J.L.; Curtis, W.R. Inhibitory role of root hairs on transport within root culture bioreactors, Biotechnology &amp Bioengineering, 70:176-186, 2000.

Mukandan, U.; Asplund, P.T.; Curtis, W.R., "Cytochemical assay to localize respiratory activity in hairy root cultures", Phytomorphology 50(2):184-187, 2000.

Curtis, W.R.; Carvalho, E.B.; Tescione, L.D. Advances and challenges in bioreactor design for the production of chemicals from plant tissue culture. Proceedings of the Fourth International Symposium on In Vitro Culture and Horticultureal Breeding (editors: Sorvari, S.; Karhu, S.; Kanervo, E.; Pihakaski, S.), presented at ISHS-International Society for Horticultural Science, Tempere, Finland, July 2-7, 2000; Acta Horticulurae, 560:247-253, 2001.

Bonser, A.M.; Berghage, R.D.; Drake, E.N.; Curtis, W.R., "Phytoremediation of aged refinery soil under saturated and unsaturated conditions", Submitted to Environmental Science &amp Technology.

Bonser, A.M.; Berghage, R.D.; Curtis, W.R.; Drake, E.N., "Phytoremediation of aged refinery soil in greenhouse and field", submitted to Environmental Science &amp Technology.

Carvalho, M.A.; Curtis, W.R., "Distillation: Laboratory Scale", In: Encyclopedia of Separation Science (Cooke, M.; Poole, C., eds.) Academic Press, London, UK, In Press for early 2000.

Curtis, W.R., "Hairy Roots, Bioreactor Growth", In: Encyclopedia of Cell Technology, Vol. 2 (Spier, R.E., ed.) John Wiley & Sons, New York, NY, pp. 827-841, 2000.

Merritt, C.D.; Surabhi, R.; Fedoroff, N.; Curtis, W.R., Direct Agrobacterium tumefaciens-mediated transformation of Hyoscyamus muticus hairy roots using green fluorescent protein, Biotechnology Progress, 15(2):278-282, 1999.

Carvalho, E.B.; Curtis, W.R., "The effect of inoculum size on growth of cell and root cultures of Hyoscyamus muticus: Implications for reactor inoculation", Biotechnology and Bioprocess Engineering, 4(4):287-293, 1999.

Tescione, L.; Asplund, P.; Curtis, W.R., "Reactor design for root culture: Oxygen mass transfer limitation", In: Plant Cell and Tissue Culture for the Production of Food Ingredients (Fu, T.-J.; Singh, G.; Curtis, W.R., eds.) Kluwer Academic / Plenum Publishing, New York, NY, pp. 139-156, 1999.

Curtis, W.R., "Achieving economic feasibility for moderate-value food and flavor additives: A perspective on productivity and proposal for production technology cost reduction", In: Plant Cell and Tissue Culture for the Production of Food Ingredients (Fu, T.-J.; Singh, G.; Curtis, W.R., eds.) Kluwer Academic / Plenum Publishing, New York, NY, pp. 225-236, 1999.

Hsiao, T.Y.; Bacani, F.T.; Carvalho, E.B.; Curtis, W.R., Development of a low capital investment reactor system: Application for plant cell suspension culture, Biotechnology Progress, 15(1): 114-122, 1999.

Ramakrishnan, D.; Luyk, D.; Curtis, W.R., Monitoring biomass in root culture systems, Biotechnology & Bioengineering,62:711-721, 1999.

Mukundan, U.; Bhide, V.; Singh, G.; Curtis, W.R., "pH-mediated release of betalains from transformed root cultures of Beta vulgaris L.", Appl. Microbiol. Biotechnol., 50: 241-245, 1998.

Carvalho, E.; Curtis, W. R., Characterization of fluid-flow resistance in root cultures with a convective flow tubular bioreactor, Biotechnology & Bioengineering, 60(3): 375-384, 1998.

Mukundan, U.; Carvalho, E.; Curtis, W.R., "Growth and pigment production by hairy root cultures of Beta vulgaris L. in a bubble column reactor", Biotechnology Letters, 20(5): 469-474, 1998.

Singh, G.; Gavrieli, J.; Oakey, J.S.; Curtis, W. R., Interaction of methyl jasmonate, wounding and fungal elicitation during sesquiterpene induction in Hyoscyamus muticus in root cultures", Plant Cell Reports, 17:391-395, 1998.

Bordonaro, J.; Carvalho, E.; Curtis, W.R. "Growth of transformed root cultures in bioreactors for the production of biochemicals", pp. 91-106, In: Proceedings of the '97 Agricultural Biotechnology Symposium on Plant Biotechnology on New Biomaterials, August 27th, Seoul National University, Suwan, Korea, The Research Center for New Bio-Materials in Agriculture (ISSN 1225-617X), 1997.

Mehmetoglu, U.; Curtis, W.R. "Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell-suspension cultures of Hyoscyamus muticus", Applied Biochemistry and Biotechnology, 67(1):71-77, 1997.

Bordonaro, J.L.; Asplund, P.T.; Curtis, W.R., "Role of root hairs in internal diffusional mass-transfer resistance in nutrient limited root tissue culture", In: Radical Biology: Advances and Perspectives on the Function of Plant Roots, (Flores, H.E.; Lynch, J.P.; Eissenstat, D. eds.), pp. 435-438, Current topics in Plant Physiology, vol. 18, American Society of Plant Physiology, Rockville, MD, 1997.

Merritt, C.; Carvalho, E.; Curtis, W.R., "Nutrient reallocation and signal transduction in root cultures exposed to pathogenic fungi", In: Radical Biology: Advances and Perspectives on the Function of Plant Roots, (Flores, H.E.; Lynch, J.P.; Eissenstat, D. eds.), pp. 458-460, Current topics in Plant Physiology, vol. 18, American Society of Plant Physiology, Rockville, MD, 1997.

Bonser, A.M.; Curtis, W.R.; Berghage, R., "Reactor design for delivery of oxygen-depleted solution to plants for measurement of root-oxygen release", In: Radical Biology: Advances and Perspectives on the Function of Plant Roots, (Flores, H.E.; Lynch, J.P.; Eissenstat, D. eds.), pp. 477-479, Current topics in Plant Physiology, vol. 18, American Society of Plant Physiology, Rockville, MD, 1997.

Bordonaro, J.L.; Curtis, W. R., "Development of a fluorescent tracer technique to evaluate mixing in plant root culture", Biotechnology Techniques, 11(8):597-600, 1997.

Tescione, L.; Ramakrishnan, D.; Curtis, W. R., "The role of liquid mixing and gas-phase dispersion in a submerged, sparged root reactor", Enzyme & Microbial Technology, 20:207-213, 1997.

Carvalho, E. B.; Holihan, S.; Pearsall, B.; Curtis, W. R. Effect of root morphology on reactor design and operation for production of chemicals. pp. 151-167, In: Hairy Roots (Doran, P. ed.) Gordon and Breach / Harwood Academic UK., 1997.

Curtis, W. R.; Wang, P.; Humphrey, A. H., "Role of calcium and differentiation in enhanced sesquiterpene elicitation from Ca-alginate immobilized plant tissue", Enzyme and Microbial Technology, 17:554-557, 1995.

Tescione, L.; Carvalho, E.; Ramakrishnan, D.; Curtis, W. R., "Oxygen transfer limitations in pilot-scale reactors for plant roots", Extended Abstracts of the Kanto (East Japan) Chapter Society of Chemical Engineers, Japan, #C214:266-267, 1995.

Ramakrishnan, D.; Curtis, W. R., "Elevated meristematic respiration in plant root cultures: implications to reactor design", Journal of Chemical Engineering of Japan, 28(4):491-493, 1995.

Singh, G.; Reddy, G. R.; Curtis, W. R., Use of binding measurements to predict elicitor dosage requirements for secondary metabolite production from root cultures, Biotechnology Progress, 10:(4):365-371, 1994.

Singh, G.; Curtis, W. R., "Growth of cell suspensions and hairy roots of Hyoscyamus muticus on different carbon sources", Life Science Advances - Plant Physiology, 13:163-168, 1994.

Ramakrishnan, D.; Salim, J.; Curtis, W. R., "Inoculation and tissue distribution in pilot-scale plant root bioreactors", Biotechnology Techniques, 8(9), pp. 639-644, 1994.

Singh, G.; Curtis, W. R. "Reactor design for plant cell suspension culture". In: Biotechnological Applications of Plant Culture, pp. 153-184 (Shargool, P.D.; Ngo, T.T., eds.) CRC Press, Boca Raton, FL, 1994. 

Singh, G.; Curtis, W. R. "Reactor design for plant root culture". In: Biotechnological Applications of Plant Culture, pp. 185-206, (Shargool, P.D.; Ngo, T.T. eds.) CRC Press, Boca Raton, FL, 1994.

Ramakrishnan, D.; Curtis, W. R. Fluid dynamic studies on plant root cultures for application to bioreactor design. In: Studies in Plant Science, 4: Advances in Plant Biotechnology, pp. 281-305 (Furusaki, S.; Ryu, D.D.Y., eds.) Elsevier, Amsterdam, 1994.

McKelvey, S. A.; Gehrig, J. A.; Hollar, K. A.; Curtis, W. R., "Growth of plant root cultures in liquid- and gas-dispersed reactor environments", Biotechnology Progress, 9(3):317-322, 1993.

Reddy, G. R.; Signs, M. W.; Flores, H. E.; Curtis, W. R., "Reversed-phase liquid chromatographic isolation of lubimin and solavetivone from Hyoscyamus muticus 'hairy' root cultures", Journal of Chromatography, 657:440-444, 1993.

Corry, J. P.; Reed, W. L.; Curtis, W. R., "Enhanced recovery of solavetivone from Agrobacterium transformed root cultures of Hyoscyamus muticus using integrated product recovery", Biotechnology and Bioengineering, 42:503-508, 1993.

Ramakrishna, S. V.; Reddy, G. R.; Curtis, W. R.; Humphrey, A. E. "Production of solavetivone by immobilized cells of Hyoscyamus muticus", Biotechnology Letters, 15(3):301-306, 1993.

Ramakrishna, S. V.; Reddy, G. R.; Curtis, W. R.; Humphrey, A. E. "Stimulation of solavetivone synthesis in free and immobilized cells of Hyoscyamus muticus by Rhizoctonia solani fungal components", Biotechnology Letters, 15(3):307-310, 1993.

Curtis, W. R.; Emery, A. H., "Plant cell suspension culture rheology", Biotechnology and Bioengineering, 42:520-526, 1993.

Curtis, W. R., "Cultivation of Roots in Bioreactors", Current Opinion in Biotechnology, 4(2):205-210, 1993.

Pannuri, S., Reddy, G. R., McNeill, D.; Curtis, W. R., "Interpreting the role of phosphorus and growth rate in enhanced fungal induction of sesquiterpenes from Hyoscyamus muticus root cultures", Applied and Microbial Biotechnology, 38:550-555, 1993.

Reddy, G. R.; Reddanna, P.; Reddy, C. C.; Curtis, W. R., "11-HEPTE is the major deoxygenation product of lipoxygenase isolated from hairy root cultures of Solanum tuberosum." Biochemical Biophysical Research Communications, 189(3):1349-1352, 1992.

Flores, H. E.; Curtis, W. R., "Approaches to understanding and manipulating the biosynthetic potential of plant roots", In: Biochemical Engineering VII: Cellular and Reaction Engineering, (Pederson, H.; Mutharsan, R.; Di Biasio, D., eds.), Proceedings of The New York Academy of Science, New York, 665:188-209, 1992.

Schnapp, S. R.; Curtis, W. R.; Hasegawa, P. M., "Estimation of growth yield and maintenance coefficient of plant cell suspensions." Biotechnology and Bioengineering, 38:1131-1136, 1991.

Schnapp, S. R.; Curtis, W. R.; Bressan, R. A. and Hasegawa, P. M., "Growth yields and maintenance of unadapted and NaCl adapted tobacco cells grown in semi-continuous culture." Plant Physiology, 96:1289-1293, 1991.

Dunlop, D. S.; Curtis, W. R., "Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation." Biotechnology Progress, 7:434-438, 1991. 

Curtis, W. R.; Hasegawa, P. M. and Emery, A. H., "Modeling linear and variable growth in phosphate limited suspension cultures of opium poppy." Biotechnology and Bioengineering, 38:371-379, 1991

Westgate, P. J.; Curtis, W. R.; Emery, A. H.; Hasegawa, P. M.; Heinstein, P. H., "Approximation of continuous growth of Cephalotaxus harringtonia plant cell cultures using fed-batch operation." Biotechnology and Bioengineering, 38:241-246, 1991.

Curtis, W. R., "Interference of intracellular phosphate analysis by phosphatase in Papaver somniferum cell suspensions." Phytochemical Analysis 1:70-73, 1990.

Others in the works

Back, K.; De Haas, C. R.; Chappell, J.; Curtis, W. R., "Sesquiterpene cyclase enzyme induction during integrated extraction of sesquiterpenes from H. muticus root cultures

Colleen's GFP signal transduction paper.

Kristin M. O’Neill, Anne M. Schilthuis, Calvin A. Leiter, Kurt M. Neihaus, Nicole A. Judge, Edda Twiddy, Alison D. O’Brien, Curtis, W.R. Scale-up of transgenic tobacco cells that express intimin of enterohemorrhagic Escherichia coli O157:H7 for use as an oral vaccine in cattle.

Pat's flourescent oxygen imaging paper

Floryn's pilot scale low cost root reactor run.

Divakar's collaborative scale-up work with Pfizer.

Mike, Tamy's & Kyung's secreted peroxidase characterization.

and many more ....


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